A potential lipid producing strain of Penicillfum sp. was isolated from natural source. Penicilliurn sp. was cultivated in an appropriate medium containing 6% of glucose as a corbon source, ammonium nitrate as a nitrogen source, C : N ratio 200, pH 4.0 for a period of 17 days at 35¡É. Under the condition, the lipid content was 64.2% of dry cell weight. The total lipid produced was 13.7g/100g of glucose consumed. The proportion of nonpolar and polar lipid fractions was 92.2% and 7.8%, respectively. The nonpolar lipid compositions of lipid produced under optimum condition were 5.3% of free fatty acids, 6.8% of free sterols, 9.3% of partial glycerides and 72.0% of triglycerides. The major fatty acids of total lipid were 20.1% of palmitic acid, 21.6% of linoleic acid and 53.3% of oleic acid.
|